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Alternatively, one can identify genes that are known to be normally methylated but, as a result of some mutation event, is no longer silenced.
en.wikipedia.org
As a result, it is reasonable to assume that any aligned mismatches were the result of a single mutation event, rather than several at the same location.
en.wikipedia.org
From such an alignment, biologists may infer shared evolutionary origins, identify functionally important sites, and illustrate mutation events.
www.medgadget.com

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